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Confocal immunofluorescent analysis of He La cells using Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit m Ab (green).
Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
This protocol is intended for immunoprecipitation of native proteins for analysis by western immunoblot or kinase activity utilizing magnetic separation.
Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.
This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our #12727 Immunofluorescence Application Solutions Kit NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
When harvesting tissue, remove unwanted material such as fat and necrotic material from the sample.
Tissue can then be processed and cross-linked immediately, or frozen on dry ice and stored at -80°C for processing later.
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Video: Program helping people with vision loss learn to use technology As Paul Saner, Commissioner of the Massachusetts Commission for the Blind put it: "This program is truly a collaboration of local and state agencies coming together for a common goal." With locations at the Brookline Senior Center, Worcester Senior Center, Worcester Public Library, and Brookes Free Library (Harwich), this FREE program is possible because of partnerships with the Massachusetts Commission for the Blind, Massachusetts Association of Councils on Aging, Executive Office of Elder Affairs, Highland Street Foundation, Boston Foundation, and the Memorial Foundation for the Blind in Worcester County.
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